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total rna template  (TaKaRa)


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    Structured Review

    TaKaRa total rna template
    Total Rna Template, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 40655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+rna+template/Mouse+Brain+Total+RNA/pmc10475957-81-0-17
    Average 96 stars, based on 40655 article reviews
    total rna template - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: (-)-Epigallocatechin-3-gallate induces interferon-λ2 expression to anti-influenza A virus in human bronchial epithelial cells (BEAS-2B) through p38 MAPK signaling pathway
    Article Snippet: Next, the PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, Dalian, China) and total RNA template was used to synthesize the cDNA.

    Reverse Transcription:

    Article Title: Transplantation of IL-1β siRNA-modified bone marrow mesenchymal stem cells ameliorates type II collagen-induced rheumatoid arthritis in rats
    Article Snippet: .. DNase I was then added to remove genomic DNA contamination at 42 ̊C for 3 min. RNA was reverse transcribed into cDNA, and the reaction components were as follows: Total RNA template (2 μg), M-MLV reverse transcriptase (Takara Biotechnology Co., Ltd.) (40 U/μl), oligo(dT) (100 nM), dNTP mix (1 mM), DTT (0.1 M), RNAse inhibitor (40 U/μl) and deionized water (RNAse-free) to a total volume of 20 μl. .. The mixture was incubated at 42 ̊C for 60 min, heated to 70 ̊C for 10 min, cooled in iced water and stored at -20 ̊C. qPCR was performed by mixing 10 μl Power SYBR Green PCR Master mix (Thermo Fisher Scientific, Inc.), cDNA (2 μl, diluted 1:20) and forward and reverse primers (0.2 μM each) to a final PCR volume of 20 μl on an ABI Prism 7700 sequence detection system (Applied Biosystems; Thermo Fisher Scientific, Inc.).

    Article Title: Long Non-Coding RNA Bmdsx-AS1 Effects on Male External Genital Development in Silkworm
    Article Snippet: .. Total RNA template was reverse transcribed into cDNA with a PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Shiga, Japan). .. The synthesized cDNA was diluted to 250 ng/μL as the template of qPCR. qPCR experiments were carried out on ABI7500 Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA).

    Article Title: LncRNA NEAT1 promotes nucleus pulposus cell matrix degradation through regulating Nrf2/ARE axis
    Article Snippet: Total RNA from nucleus pulposus cells was extracted using Trizol reagent (Vazyme, Nanjing, China). .. Total RNA template (500 ng) was reverse-transcribed into cDNA using a PrimeScript RT Master Mix Kit (RR036, Takara, Kyoto, Japan). .. Then, quantitative real-time RT-PCR (qRT-PCR) was performed on Roche LightCycler 96 System (CT, USA) using SYBR Premix EX TaqTM (RR420A, TakaRa, Kyoto, Japan).

    Article Title: MaABI5 and MaABF1 transcription factors regulate the expression of MaJOINTLESS during fruit abscission in mulberry ( Morus alba L.)
    Article Snippet: .. Total RNA template was reverse transcribed into cDNA with a PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa, Shiga, Japan). .. The synthesized cDNA was diluted to 200 ng/μL as the template for qPCR. qPCR experiments were carried out on ABI7500 Real-Time PCR machine (Applied Biosystems, Foster City, CA, USA).

    Synthesized:

    Article Title: The functional difference of eight chitinase genes between male and female of the cotton mealybug, Phenacoccus solenopsis.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved.. The cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a polyphagous insect that attacks tens of plant and causes substantial economic loss.

    Article Title: Magnetopriming Actuates Nitric Oxide Synthesis to Regulate Phytohormones for Improving Germination of Soybean Seeds under Salt Stress
    Article Snippet: The integrity of all RNA samples was confirmed on 1.2% ( w / v ) denaturing agarose gel electrophoresis followed by RNA quantification using NanoDropTM 2000 (Thermo Scientific, Waltham, MA, USA). .. Following this, the cDNA was synthesized with approximately 2 μg total RNA template and oligo (dT) 18 primer by using PrimeScriptTM 1st strand cDNA Synthesis Kit (TaKaRa Kusatsu, Japan) following manufacturer’ s instructions. .. The qRT-PCR reaction (10 μL) was set using PowerUp TM SYBR TM green master mix (Applied Biosystem, Waltham, MA, USA) and was performed in StepOnePlus TM Real-time PCR system (Applied Biosystem).

    cDNA Synthesis:

    Article Title: The functional difference of eight chitinase genes between male and female of the cotton mealybug, Phenacoccus solenopsis.
    Article Snippet: A cc ep te d A rt ic le This article is protected by copyright.. All rights reserved.. The cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is a polyphagous insect that attacks tens of plant and causes substantial economic loss.

    Article Title: Magnetopriming Actuates Nitric Oxide Synthesis to Regulate Phytohormones for Improving Germination of Soybean Seeds under Salt Stress
    Article Snippet: The integrity of all RNA samples was confirmed on 1.2% ( w / v ) denaturing agarose gel electrophoresis followed by RNA quantification using NanoDropTM 2000 (Thermo Scientific, Waltham, MA, USA). .. Following this, the cDNA was synthesized with approximately 2 μg total RNA template and oligo (dT) 18 primer by using PrimeScriptTM 1st strand cDNA Synthesis Kit (TaKaRa Kusatsu, Japan) following manufacturer’ s instructions. .. The qRT-PCR reaction (10 μL) was set using PowerUp TM SYBR TM green master mix (Applied Biosystem, Waltham, MA, USA) and was performed in StepOnePlus TM Real-time PCR system (Applied Biosystem).



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    Comparison of T cell receptor (TCR) gene repertoire clonality among metastatic lesions in a melanoma case. The percentages of the top 50 clones from three metastatic lesions and non-cancerous tissues (normal) are shown. TCR repertoire sequencing data were obtained using a human TCRα and TCRβ profiling kit and the Miseq NGS system. TCR repertoire characterization was performed using the MiXCR software (http://mixcr.milaboratory.com/). TCR: T cell receptor; nor: normal; meta: metastatic.

    Journal: Cancer Genomics & Proteomics

    Article Title: Characterization of the Neoantigen Profile in a Tumor Mutation Burden-high Melanoma Patient With Multiple Metastases

    doi: 10.21873/cgp.20517

    Figure Lengend Snippet: Comparison of T cell receptor (TCR) gene repertoire clonality among metastatic lesions in a melanoma case. The percentages of the top 50 clones from three metastatic lesions and non-cancerous tissues (normal) are shown. TCR repertoire sequencing data were obtained using a human TCRα and TCRβ profiling kit and the Miseq NGS system. TCR repertoire characterization was performed using the MiXCR software (http://mixcr.milaboratory.com/). TCR: T cell receptor; nor: normal; meta: metastatic.

    Article Snippet: Total RNAs were isolated from three metastatic tumors (rib, intra-muscular, and brain lesions) and subjected to the Switching Mechanisms at 5’ End of RNA Template (SMARTer TM ) human TCRα and TCRβ profiling kit (Clontech Laboratories Inc., Mountain View, CA, USA) and Miseq NGS system (Illumina Inc., San Diego, CA, USA) as previously reported ( ).

    Techniques: Comparison, Clone Assay, Sequencing, Software

    Comparison of TCR gene repertoire parameters among metastatic lesions in a melanoma case. DE 50 values (A) and the Shannon index (B) were calculated and compared among three metastatic lesions and non-cancerous (normal)tissues. TCR: T cell receptor.

    Journal: Cancer Genomics & Proteomics

    Article Title: Characterization of the Neoantigen Profile in a Tumor Mutation Burden-high Melanoma Patient With Multiple Metastases

    doi: 10.21873/cgp.20517

    Figure Lengend Snippet: Comparison of TCR gene repertoire parameters among metastatic lesions in a melanoma case. DE 50 values (A) and the Shannon index (B) were calculated and compared among three metastatic lesions and non-cancerous (normal)tissues. TCR: T cell receptor.

    Article Snippet: Total RNAs were isolated from three metastatic tumors (rib, intra-muscular, and brain lesions) and subjected to the Switching Mechanisms at 5’ End of RNA Template (SMARTer TM ) human TCRα and TCRβ profiling kit (Clontech Laboratories Inc., Mountain View, CA, USA) and Miseq NGS system (Illumina Inc., San Diego, CA, USA) as previously reported ( ).

    Techniques: Comparison